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מתמטיקה פיננסים בריאות וכושר תאריך ושעה ממירי יחידות מחשב פיזיקה כימיה בנייה הנדסה סטטיסטיקה חינוך חיי יומיום ביולוגיה רכב בישול ומזון ספורט וכושר נדל"ן צילום חשמל חקלאות נסיעות עסקים
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מחשבון מספר עותקים

מספר מולקולות DNA ממסה ומאורך הקטע.

מבוא

Copy Number Calculator — DNA molecule count from mass and fragment length. Enter DNA mass (ng), Fragment length (bp) to get an instant, accurate result.

נוסחה

Molecule count = (mass in ng × Avogadro's number 6.022×10²³) / (length in bp × 650 g/mol/bp × 10⁹) — converts a mass of dsDNA into an absolute number of DNA molecules (copies), using the ~650 g/mol/bp approximation for molecular weight.

צעד אחר צעד

  1. Enter the DNA mass (ng).
  2. Enter the Fragment length (bp).
  3. Click Calculate to see your result instantly.

דוגמה מהעולם האמיתי

Example: With DNA mass (ng) = 100, Fragment length (bp) = 3000, the Copy Number Calculator gives Molecules: 30882051282.0513.

שאלות נפוצות

Why is Avogadro's number used here?
Avogadro's number (6.022×10²³ molecules per mole) is the conversion factor between moles of DNA and an actual molecule count, once the mass has been converted to moles using the molecular weight.
What is this typically used for?
Commonly for qPCR standard curve preparation or NGS library quantification, where you need to know the exact number of DNA copies (not just the mass) in a known-concentration sample.
What inputs does the Copy Number Calculator need?
You'll need to provide: DNA mass (ng), Fragment length (bp). All fields use sensible defaults, so you can see a working example immediately and then adjust the values to match your own numbers.
How accurate is the Copy Number Calculator?
The Copy Number Calculator applies the formula exactly as calculated — Molecule count = (mass in ng × Avogadro's number 6.022×10²³) / (length in bp × 650 g/mol/bp × 10⁹) — converts a mass of dsDNA into an absolute number of DNA molecules (copies), using the ~650 g/mol/bp approximation for molecular weight. — so results are precise for the inputs you provide. Accuracy depends on entering correct, realistic input values.
Is the Copy Number Calculator free to use?
Yes, the Copy Number Calculator is completely free, requires no signup, and runs instantly in your browser.

אודות מחשבון מספר עותקים

The Copy Number Calculator uses a real, verifiable formula — Molecule count = (mass in ng × Avogadro's number 6.022×10²³) / (length in bp × 650 g/mol/bp × 10⁹) — converts a mass of dsDNA into an absolute number of DNA molecules (copies), using the ~650 g/mol/bp approximation for molecular weight. — so results are accurate every time, not an approximation.