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Calcolatore del numero di copie del plasmide

Numero di molecole di plasmide da massa e lunghezza del plasmide.

Introduzione

Plasmid Copy Number Calculator — Plasmid molecule count from mass and plasmid length. Enter Plasmid mass (ng), Plasmid length (bp) to get an instant, accurate result.

Formula

Uses the same molecule-count formula as the general copy number calculator: molecules = (mass in ng × Avogadro's number) / (plasmid length in bp × 650 g/mol/bp × 10⁹) — framed specifically for plasmid mass and length inputs.

Passo dopo passo

  1. Enter the Plasmid mass (ng).
  2. Enter the Plasmid length (bp).
  3. Click Calculate to see your result instantly.

Esempio pratico

Example: With Plasmid mass (ng) = 100, Plasmid length (bp) = 5000, the Plasmid Copy Number Calculator gives Molecules: 18529230769.2308.

Domande Frequenti

How is this different from the general copy number calculator?
It uses the identical formula — this version is simply presented with plasmid-specific labeling (plasmid mass and plasmid size in bp) since plasmid copy number is one of the most common uses of this calculation, e.g. before a transformation or ligation.
Should I use the full plasmid length including the insert?
Yes, enter the total size of the plasmid construct in base pairs (backbone plus any cloned insert), since that determines its actual molecular weight.
What inputs does the Plasmid Copy Number Calculator need?
You'll need to provide: Plasmid mass (ng), Plasmid length (bp). All fields use sensible defaults, so you can see a working example immediately and then adjust the values to match your own numbers.
How accurate is the Plasmid Copy Number Calculator?
The Plasmid Copy Number Calculator applies the formula exactly as calculated — Uses the same molecule-count formula as the general copy number calculator: molecules = (mass in ng × Avogadro's number) / (plasmid length in bp × 650 g/mol/bp × 10⁹) — framed specifically for plasmid mass and length inputs. — so results are precise for the inputs you provide. Accuracy depends on entering correct, realistic input values.
Is the Plasmid Copy Number Calculator free to use?
Yes, the Plasmid Copy Number Calculator is completely free, requires no signup, and runs instantly in your browser.

Informazioni su Calcolatore del numero di copie del plasmide

The Plasmid Copy Number Calculator uses a real, verifiable formula — Uses the same molecule-count formula as the general copy number calculator: molecules = (mass in ng × Avogadro's number) / (plasmid length in bp × 650 g/mol/bp × 10⁹) — framed specifically for plasmid mass and length inputs. — so results are accurate every time, not an approximation.